Figure 4
From: Refractoriness of hepatitis C virus internal ribosome entry site to processing by Dicer in vivo
Dicer does not bind HCV IRES in vivo. HCV IRES nt 1-341 was amplified by RT-PCR from RNA extracted from Dicer immunoprecipitates (IPs) prepared from Huh-7 or 9–13 cells by ribonucleoprotein immunoprecipitation (RIP) assay. The amplified DNA products were analyzed by 1.5% agarose gel electrophoresis and stained with ethidium bromide (lower panel). Proteins (100 μg) were analyzed by 10% SDS-PAGE to visualize Dicer protein expression or immunoprecipitation in Huh-7 and 9–13 cells (upper panel).