Figure 2
From: Refractoriness of hepatitis C virus internal ribosome entry site to processing by Dicer in vivo
Recombinant Dicer binds and cleaves HCV IRES in vitro. (A-B) Electrophoretic mobility shift assays (EMSA) 32P-labeled HCV RNA nt 1-341 (A) or nt 1-515 (B) was incubated in the absence or presence of recombinant human Dicer (200 ng) and/or BSA (2 μg), and complex formation visualized by non-denaturing PAGE and autoradiography. (C-D) Dicer RNase activity assays. (C) 32P-labeled HCV RNA nt 1-341 (left panel) or nt 1-515 (right panel) was incubated in the absence (-) or presence (+) of recombinant human Dicer (200 ng), and HCV RNA processing monitored by denaturing PAGE and autoradiography. Lanes 4, 5, 6 and 7 represent higher numerical exposition of lanes 2, 3, 8 and 9 respectively. M, indicates a 10-nt RNA size marker.