Table 1 RT-PCR primer sequences, product sizes and annealing temperatures
From: Human spongiosa mesenchymal stem cells fail to generate cardiomyocytes in vitro
Gene | Primer sequence | Product size, bp | Annealing temperature, °C |
|---|---|---|---|
hMYH7B | 5'-AGCAGAAGCGCAACGCAGAGT-3' 5'-TGCTGCACCTTGCGGAACTTG-3'- | 217 | 60 |
hMEF-2D | 5'-TGCCCACTGCCTACAACACAG-3' 5'-GGACACCGGTTCTGACTTGATG-3' | 347 | 58 |
hMEF-2A | 5'-GCAGCAGCACCACCTAGGAC-3' 5'-CTGCTGCTGCTGCTGGAAG-3' | 172 | 58 |
hTnI | 5'-ACTTGTGCCGACAGCTCCAC-3' 5'-CCTTCTTCACCTGCTTGAGGTG-3' | 252 | 58 |
hANP | 5'-CAACGCAGACCTGATGGATTTC-3' 5'-AGATGACACGAATGCAGCAGAG-3' | 452 | 58 |
hNkx2.5 | 5'-CAAGGACCCTAGAGCCGAAAAG-3' 5'-CCTGCGTGGACGTGAGTTTC-3' | 240 | 58 |
hGAPDH | 5'-GAAGGTGAAGGTCGGAGTC-3' 5'-GAAGATGGTGATGGGATTTC-3' | 235 | 58 |