Figure 5
From: Human spongiosa mesenchymal stem cells fail to generate cardiomyocytes in vitro
Expression of cardiac-specific genes by spongiosa hMSCs cultured in DC on biomaterials. (A) PCR from spongiosa hMSCs cultured on biomaterials demonstrating the effectiveness of RG503 and Texin 950 as scaffolds for supporting of cardiac differentiation of hMSCs in vitro. (B) Clusterization of cell cultures grown on different biomaterials based on the level of MYH7B, Nkx2.5, MEF2A, and MEF2D gene expression. The cell cultures tested in this experiment formed two clusters at Euclidian distance of about 2.8. The level of dissimilarity between these clusters was found to be at the edge of statistical significance (P = 0.06, ANOSIM). Note that the cells cultured on biomaterials RG503 and Texin 950 formed a separate cluster. (TCPS = tissue culture polystyrene).