Lack of association between mutations of gene-encoding mitochondrial D310 (displacement loop) mononucleotide repeat and oxidative stress in chronic dialysis patients in Taiwan

Table 2 Oxidative stress markers, and mtDNA copy number according to D310 mononucleotide repeat

	D310 repeat groups	Dialysis patients (N= 184)	Control group (N= 213)	P-value
7-C		n = 70	n = 67
	TBARS (μM/L)	1.110 ± 0.633	0.763 ± 0.423	0.000
	Free thiol (μM/L)	1.463 ± 0.460	2.185 ± 0.450	0.000
	mtDNA copy number	1638 ± 2263	423 ± 295	0.000
8-C		n = 65	n = 9
	TBARS (μM/L)	1.205 ± 0.635	0.797 ± 0.530	0.000
	Free thiol (μM/L)	1.500 ± 0.461	2.064 ± 0.420	0.000
	mtDNA copy number	1783 ± 2602	416 ± 263	0.000
9-10C		n = 45	n = 47
	TBARS (μM/L)	1.060 ± 0.625	0.752 ± 0.535	0.013
	Free thiol (μM/L)	1.607 ± 0.424	2.099 ± 0.396	0.000
	mtDNA copy number	1594 ± 3023	374 ± 257	0.007
T-to-C transition		n = 4	n = 7
	TBARS (μM/L)	0.863 ± 0.454	0.634 ± 0.199	0.268
	Free thiol (μM/L)	1.630 ± 0.704	2.060 ± 0.356	0.203
	mtDNA copy number	933 ± 725	499 ± 215	0.161

Data (means ± SD) on post hoc analysis of TBARS, free thiol, and mtDNA copy number in same group, either dialysis or control groups. All were nonsignificant. TBARS, thiobarbituric acid reactive substances; mtDNA, mitochondrial DNA.
Correlation of D310 mononucleotide repeat and oxidative stress markers, mtDNA copy number. The study subjects were categorized into 4 subgroups depending on D310 alterations, and significant differences were observed in the levels of oxidative stress biomarkers and mtDNA copy number between the dialysis patients and the control subjects of the 4 subgroups. Analysis of the variables by ANOVA with the post hoc test based on the LSD method revealed that a difference in the D310 alterations (7-C to 10-C) did not result in significant difference in the levels of oxidative stress biomarkers and mtDNA copy number in the dialysis patients.

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