Table 5 Overview of culturing and recording conditions used by other researchers. The following is a synopsis of conditions reported in the Methods section of the papers listed in Table 1.

Ref.

T (°C)

Perfusion?

pH

Age at time of Expts.

Culture Medium

Ca²⁺

Mg²⁺

K⁺

Osmolarity (mOsm)

Plating Density (/mm²)

Observed Density (/mm²)

Type of Stim.

[24]

20

Y

7.2

1–6 w

DMEM-5/5

1

0–2

2.8

330–334

10, 000

-

V

[25]

-

-

7.2

1–5 w

DMEM-5/5

1

1–10

2.8

336–354

-

-

V

[26]

-

-

-

30–50 d

DMEM-5/5

-

-

-

-

-

120

V

[27]

-

Y

7.2

40–50 d

DMEM-5/5

2

1

2.8

334

-

175

V

[3]

37

-

-

3 w

MEM-5HS

-

-

-

-

-

300

I

[5]

37

-

-

3 w–3 m

MEM-5FBS

1.8

0.8

5.4

330

8000

-

V

This paper

35

N

7.2

10–32 d

DMEM-10HS

1.8

0.8

5.4

330

2700

2500

V