From: Searching for plasticity in dissociated cortical cultures on multi-electrode arrays
Protocol | Tetanus | Probing | Conditions | No. and ages of cultures | Total Expts. | Intervals |
---|---|---|---|---|---|---|
III.1 | 20 trains (ITI: 2 s) of 20 pulse pairs (IPI: 50 ms; IEI: 5 ms). | Single pulses of 0.8 V, at 5 s intervals. | Baseline medium, with spontaneous bursts. | 4; 13–16 div | 8 | 2 h |
III.2 | 20 trains (ITI: 2 s) of 20 pulse pairs (IPI: 50 ms; IEI: 5 ms). | Single pulses of 0.8 V, at 5 s intervals. | Electrical burst quieting as in II.2. | 3a; 13–16 div | 6 | 2 h |
III.3 | 20 trains (ITI: 2 s) of 20 pulse pairs (IPI: 50 ms; IEI: 5 ms). | Single pulses of 0.8 V, at 5 s intervals. | Spontaneous bursts suppressed by 1 mM magnesium. | 4; 25–28 div | 8 | 2 h |
III.4 | 150 trains (ITI: 6 s) of 20 pulse pairs (IPI: 100 ms; IEI: 10 ms). | Single pulses of 0.6 V, at 1 s intervals. | Electrical burst quieting as in II.2, but not suspended during tetanization. | 4; 20–23 div | 8 | 2 h |