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Table 4 Details of experiments on plasticity induced in specific responses (Series III)

From: Searching for plasticity in dissociated cortical cultures on multi-electrode arrays

Protocol

Tetanus

Probing

Conditions

No. and ages of cultures

Total Expts.

Intervals

III.1

20 trains (ITI: 2 s) of 20 pulse pairs (IPI: 50 ms; IEI: 5 ms).

Single pulses of 0.8 V, at 5 s intervals.

Baseline medium, with spontaneous bursts.

4; 13–16 div

8

2 h

III.2

20 trains (ITI: 2 s) of 20 pulse pairs (IPI: 50 ms; IEI: 5 ms).

Single pulses of 0.8 V, at 5 s intervals.

Electrical burst quieting as in II.2.

3a; 13–16 div

6

2 h

III.3

20 trains (ITI: 2 s) of 20 pulse pairs (IPI: 50 ms; IEI: 5 ms).

Single pulses of 0.8 V, at 5 s intervals.

Spontaneous bursts suppressed by 1 mM magnesium.

4; 25–28 div

8

2 h

III.4

150 trains (ITI: 6 s) of 20 pulse pairs (IPI: 100 ms; IEI: 10 ms).

Single pulses of 0.6 V, at 1 s intervals.

Electrical burst quieting as in II.2, but not suspended during tetanization.

4; 20–23 div

8

2 h

  1. aIn a 4th culture, burst suppression did not work sufficiently well. Those data were excluded from further analysis.