From: Searching for plasticity in dissociated cortical cultures on multi-electrode arrays
Protocol | Tetanus target | Probe amplitude | Conditions | No. and ages of cultures | Total expts. | Intervals |
---|---|---|---|---|---|---|
II.1 | Single electrode. | Fixed, 0.8 V. | Baseline medium, spontaneous bursting. | 4; 17–22 div | 8 | 2 h |
II.2 | Single electrode. | Fixed, 0.8 V. | Bursts completely suppressed by 50 Hz background stimulation distributed over 20–40 electrodes, except during tetanization. | 3a; 17–22 div | 6 | 2 h |
II.3 | Single electrode. | Fixed, 0.8 V. | Spontaneous bursts suppressed by 1 mM magnesium. | 3; 26–28 div | 6 | 2 h |
II.4 | Single electrode. | Reduced (see Methods). | Spontaneous bursts suppressed by 2 mM magnesium. | 4; 29–32 div | 16 | 2 h |
II.5a | 8 electrodes, as in I.3a. | Range of voltages, 100–900 mV. | Spontaneous bursts suppressed by 1–2 mM magnesium. | 3; 18–20 div | 12 | 2 h |
II.5b | 8 electrodes, as in I.3b. | Range of voltages, 100–900 mV. | Spontaneous burst suppressed by 2 mM magnesium. | 1; 17 div | 4 | 2 h |