From: Searching for plasticity in dissociated cortical cultures on multi-electrode arrays
Protocol | Tetanus | Conditions | No. and ages of cultures | Total expts. | Intervalsa |
---|---|---|---|---|---|
I.1 | Sets of 4 volleys (IVI: 500 ms) to 10 geometrically close electrodes (IEI: 5 ms), repeated every 5 s for 15 min. | Baseline medium, spontaneous bursting. | 2 × 2 b; 10–19 div | 4 | - |
I.2 | Single volleys to 5 electrodes (IEI: 2 ms), repeated every 10 s for 17 min. | Baseline medium, spontaneous bursting. | 4; 13–16 div | 16 | 4 h |
I.3a | Single volleys to 8 electrodes in a vertical column (IEI: 2 ms), repeated every 10 s for 15 min. | Elevated magnesium (1–2 mM) to reduce spontaneous bursting. | 3; 18–20 div | 6 | 2 h |
I.3b | Sets of 20 volleys (IVI: 50 ms) to 8 electrodes in a vertical column (IEI: 2 ms), repeated every 5 s for 8 min. | Elevated magnesium (1–2 mM) to reduce spontaneous bursting. | 1; 17 div | 2 | 2 h |