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Table 2 Details of experiments on plasticity expressed in burst patterns (Series I).

From: Searching for plasticity in dissociated cortical cultures on multi-electrode arrays

Protocol

Tetanus

Conditions

No. and ages of cultures

Total expts.

Intervalsa

I.1

Sets of 4 volleys (IVI: 500 ms) to 10 geometrically close electrodes (IEI: 5 ms), repeated every 5 s for 15 min.

Baseline medium, spontaneous bursting.

2 × 2 b; 10–19 div

4

-

I.2

Single volleys to 5 electrodes (IEI: 2 ms), repeated every 10 s for 17 min.

Baseline medium, spontaneous bursting.

4; 13–16 div

16

4 h

I.3a

Single volleys to 8 electrodes in a vertical column (IEI: 2 ms), repeated every 10 s for 15 min.

Elevated magnesium (1–2 mM) to reduce spontaneous bursting.

3; 18–20 div

6

2 h

I.3b

Sets of 20 volleys (IVI: 50 ms) to 8 electrodes in a vertical column (IEI: 2 ms), repeated every 5 s for 8 min.

Elevated magnesium (1–2 mM) to reduce spontaneous bursting.

1; 17 div

2

2 h

  1. aBetween experiments on a single culture.
  2. bTwo cultures were each used twice, 6 days apart, resulting – for practical purposes – in four independent experiments.