Table 2 PCR primer and TaqMan® Probe Sequences
From: Polymorphisms within inflammatory genes and colorectal cancer
Gene | Rs number | Trivial name | Primer Forward | Primer Reverse | VIC Probe | 6FAM Probe |
|---|---|---|---|---|---|---|
TLR4 | rs4986790 | Asp299Gly | CCATTGAAGAATTCCGATTAGCATA | CACTCACCAGGGAAAATGAAGAA | CCTCGATGATATTATT | CTCGATGGTATTATTG |
CD14 | rs2569190 | -260 C>T | GGAAATATTGCAATGAAGGATGTTT | CTAGATGCCCTGCAGAATCCTT | CTGTTACGGTCCCC | TGTTACGGCCCCC |
MCP1 | rs1024611 | -2518 A>G | GGGAGGGCATCTTTTCTTGAC | GGTGAAGGGTATGAATCAGAAAAGA | ACAGCTATCACTTTC | AGACAGCTGTCACTTT |
IL12A | rs2243138 | +7506 A>T | AACCAGGAGTCCCCGATCC | GGCCCACTGCCCAACAG | CCATGGAGTGGTACTG | AGGCCATGGTGTGGT |
IL12A | rs668998 | +8707 A>G | CCAATCTTTCTCCCTGTAAATGTGTT | TGAATGTCTAATAGGGCAAGAATTTG | TGCATGACAAACAT | CTGCATGGCAAACA |
IL12A | rs2243151 | +9177 A>T | TTATTGCATGGTTAGTTTTTCACTTTTT | GGAGCATTGGAATGATTTGGTT | AGCATACTACACTACTTG | AGCATACTACACAACTT |
IL12A | rs2133310 | +9508 G>A | GGGACCTAATTAACTGTGTTATTGTGA | AGCGGGTGTTCTGATGTCTTG | AGTGCCAACACCTAG | TGCCAGCACCTAGT |
NOS2A | rs944722 | +524G>A | CTCTGTTTCTCTGATCCCACTTTCT | GTGATATGAGATCTCGCCACTACACT | TGGAGTCTCTGTCACC | ATGGAGTTTCTGTCACC |
TNF | rs1799724 | -857C>T | GGTAGGAGAATGTCCAGGGCTAT | AGGTCCTGGAGGCTCTTTCACT | CCCTGTCTTCGTTAAG | CCCTGTCTTCATTAAG |
PTGS1 | rs5794 | V444I | GCATGAAACCCTACACCTCCTT | GGGACTGCATCCAGGAAACA | AGGAGCTCGTAGGTGA | CAGGAGCTCATAGGT |