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Figure 3 | Journal of Negative Results in BioMedicine

Figure 3

From: Wnt expression is not correlated with β-catenin dysregulation in Dupuytren's Disease

Figure 3

Comparison of Wnt expression in RNA derived from tissue, FPCL and monolayer culture. Comparisons of the in vivo and in vitro expression levels of Wnts 5a, 9a, 10b, and 11 were performed by RT-PCR using the Wnt-specific oligonucleotide primers from RNA derived from DD and PF control samples. As shown, a distinct expression hierarchy (Tissue > FPCL > monolayer) was shared by Wnts 9a, 10b and 11 with Wnts 9a and 10b being undetected in monolayer culture. Wnt5a expression levels were unaffected by culture conditions. In all cases Wnt expression was normalized to GAPDH expression and represent the mean value ± standard deviation.

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