Figure 2
From: Wnt expression is not correlated with β-catenin dysregulation in Dupuytren's Disease
Degenerate primer design. Degenerative Wnt primers (designated Upper and Lower Primers) were designed using the ClustalW program with the aid of the BioEdit sequence alignment editor. Comparison of the published nucleic acid sequences for the 13 human Wnts detected by Affymetrix Microarray revealed two highly conserved regions that were used to design degenerate oligonucleotide primers for PCR amplification as shown.