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Table 1 Transfection efficiency (% of live cells) in mature osteoclast cultures

From: Unsatisfactory gene transfer into bone-resorbing osteoclasts with liposomal transfection systems

 

1 h

24 h

48 h

5 d

Baseline

0

0

0,12 ± 0,042

0

Adenovirus

1,2 ± 0,095

9,7 ± 1,4

15,2 ± 3,4

3,8 ± 0,96

T1: Metafectene

0

0

0

0

T2: Lipofectamine plus

0

0

0

0

T3: Tfx-50

0

0

0

0

T4: FuGene6

0

0

0

0

T5: DOTAP

0

0

0

0

T6: DOSPER

0

0

0

0

T7: JetPei

0

0

0

0

T8: DuoFect

0

0

0

0

  1. GFP-expressing and negative osteoclasts were counted using fluorescence microscopy and phase optics, and transfection efficiencies were counted. ANOVA: p = 1,4 × 10-8, n = 5.