Figure 5
From: Unsatisfactory gene transfer into bone-resorbing osteoclasts with liposomal transfection systems
Viability index in osteoclast cultures. Cells were treated with transfection reagents for 2 h, followed by culture for 4 h, 8, or 24 h. Osteoclast differentiation cultures were performed on collagen-coated plates to allow the use of the microplate reader. After transfection, cells were stained with Calcein AM and EthD and fluorescence of the dyes was measured using appropriate band pass filters. BL, baseline with no additions; Ad, adenoviral infection of GFP; T1-T8, transfection reagents as shown in Tables 1 and 2. ANOVA: p < 0,001