Figure 1
From: Unsatisfactory gene transfer into bone-resorbing osteoclasts with liposomal transfection systems
Visualization of actin rings and TRACP-positive cells in osteoclast cultures. Osteoclasts were differentiated in the presence of RANKL, M-CSF and TGF-β1 for 7 days, followed by fixation and staining of actin rings (a-c) and TRACP (d). Baseline control is shown in a and d, and adenovirus-infected cells 4 h post infection are shown in b. A typical view of the cells incubated 2 h with transfection reagents and 4 h in fresh medium is shown in c.