Figure 1

Fluorescent immunostaining of cortactin in isolated rat Sertoli cells plated on Matrigel^®. Sertoli cell monocultures were incubated without (A,C,E) and with (B,D,F) latex beads for 15 min (A,B), 2 hr (C,D), and 24 hr (E,F) and immunostained for cortactin. The negative staining control (A inset) was a primary antibody deletion. Cortactin = green; latex beads = red; Sertoli cell nuclei = blue (arrows also indicate this). Bar = 10 μm.