Figure 6

WT1 isoform A does not regulate expression of GP210. SAOS cells conditionally expressing WT1 isoform A were grown in the presence (Lanes 1–3) or absence (lane 4–6) of tetracycline to induce expression of WT1. Triplicate plates of cells were harvested for total RNA and subjected to sequential northern blot analysis with the GP210 probe, WT1probe and actin control.