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Figure 1 | Journal of Negative Results in BioMedicine

Figure 1

From: Fusion of green fluorescent protein to the C-terminus of granulysin alters its intracellular localization in comparison to the native molecule

Figure 1

Granulysin-GFP fusion protein is expressed and secreted but doesn't colocalize with perforin. a) Immunoblot analysis using anti-GFP sera was performed on cell lysate and cell media supernatant samples of YT cells expressing granulysin-GFP fusion protein (YT.granGFP) and native GFP (YT.GFP). b) Confocal immunofluorescence staining for granulysin (green) and perforin (red) was performed for YT expressing native granulysin (YT.granulysin) and granulysin-GFP fusion protein (YT.granGFP).

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