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Figure 5 | Journal of Negative Results in BioMedicine

Figure 5

From: A heparin binding synthetic peptide from human HIP / RPL29 fails to specifically differentiate between anticoagulantly active and inactive species of heparin

Figure 5

Binding Curves of GAGs to HIP Peptide-1. Dilutions of Hp, CS-E, DS, CS-C or Bovine kidney-HS (BK-HS) were added in 400 μl PBS to 100 μl of a 1:1 slurry of HIP peptide-1 sepharose : PBS. After a 10-minute incubation, the tubes were washed with three serial dilutions of 500 μl PBS. Bound materials were eluted with 2 M NaCl and quantified as in "Materials and Methods." Values shown are the total amounts bound (Y-axis) versus the concentration of the material added (X-axis). Hp, CS-E, and DS were fitted to a logarithmic curve with r squared values of 0.81 or greater.

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