Figure 2
From: Circulating rotaviral RNA in children with rotavirus antigenemia
Genomic RNA protection assay by treatment with benzonase endonuclease. Agarose gel electrophoresis shows results of VP6 gene amplification by RT-PCR after treating genomic RNA and intact virus particles with benzonase endonuclease. Lane 1, 100 bp molecular weight marker; genomic RNA of different concentrations (7.3 × 107, 9.0 × 105 and 8.5 × 103) of rotavirus SA11 strain were untreated (lanes 2, 4 and 6) and treated (lanes 3, 5 and 7) with 100 U of benzonase, different concentrations (7.3 × 107, 9.0 × 105 and 8.5 × 103) of rotavirus SA11 strain were untreated (lanes 8, 10 and 12) and treated (lanes 9, 11 and 13) with 100 U of benzonase, and lane 14 contains negative control. Amplicons of VP6 (479 bp) are present in lanes 2, 4 and 6 and absent in lanes 3, 5 and 7, indicating that in these lanes, genomic RNA is degraded by benzonase endonuclease. Amplicons are present in lanes 8–13, indicating that genomic RNA is protected when intact rotavirus particles are treated with benzonase endonuclease.