Localisation and semi quantification of c-kit ligand staining in fetal testes collected from ewes on 100% maintenance (control: C, n = 6) and 50% maintenance (L) diets (n = 9). c-kit (A) was localised to the cord germ cells (arrows) and interstitial area. Staining intensity (B) and area stained (C) were visually assessed by a single observer using an arbitrary four point scale. The cords and interstitial areas were assessed independently. No significant differences were observed between C and L testes. Scale bar (A) = 50 μM. Inset (A) = IgG negative control. Values (B,C) were expressed as means ± SEM. Where no error bar is given, all visually assessed values were identical.