Localisation and percentages of nucleated areas stained for Mcl-1 (A,B) and Bax (C,D) in day 110 fetal testes. Mcl-1 (anti-apoptosis) (A) and its antagonist Bax (C) are predominant in the germ cells (arrows). No significant differences were observed between testes from C ewes (100% maintenance diet: n = 6) and L ewes ( 50% maintenance diet: n = 9) ewes. Scale bar = 50 μM. Inset = IgG negative control. Values are expressed as means ± SEM.