Sertoli cell numbers and the expression of Ki67 in testes of day 110 sheep fetuses from ewes fed 100% maintenance requirements (control C: n = 6) or 50% maintenance requirements (Low; L; n = 9) diets. (A) Fetal testis Sertoli cell numbers, (B) localisation of the proliferation marker Ki67; arrows depict positive Ki67 nuclear staining (C) Numbers of Ki67 positive cells counted in the seminiferous cords and in the interstitial area. No significant differences were observed between C and L testes. Values are expressed as means ± SEM. In B, scale bar = 50 μM. Inset = IgG negative control.