Figure 6

Rapamycin stabilizes FRB^PLF-CFP in cultured DRG neurons but does not induce translocation of Venus-FKBP12-Inp54p. A) Confocal images of cultured DRG neurons from Rosa-FRB^PLF/CGRP-Inp54p double heterozygous mice after culturing for 24 hours in presence of vehicle or 1 μM rapamycin. B) Quantification following vehicle/rapamycin treatment. While the ratio of FRB^PLF-CFP increases after rapamycin treatment, this is due to C) an increase in FRB^PLF-CFP fluorescence, rather than translocation. n = 86 neurons per condition ***P < 0.0001. Scale bar, 20 μm.