Figure 5

Short-term rapamycin treatment does not induce translocation of Venus-FKBP12-Inp54p in cultured DRG neurons. A) Cultured DRG neurons from male Rosa-FRB^PLF/CGRP-Inp54p mice were plated for 24 hours. B) 1 μM rapamycin was applied for 10 minutes, and post-rapamycin images were taken. C) Close-up (5x zoom) of box region in (A) to show pre-rapamycin localization, and D) 5x zoom of box region in (B) to demonstrate the lack of Inp54p enrichment to FRB-tagged membrane after rapamycin treatment. A-D show FRB^PLF-CFP localization, A’-D’ demonstrate Venus-FKBP12-Inp54p localization, and the merge is shown in A”-D”.