Skip to main content
Figure 2 | Journal of Negative Results in BioMedicine

Figure 2

From: Biological constraints limit the use of rapamycin-inducible FKBP12-Inp54p for depleting PIP2 in dorsal root ganglia neurons

Figure 2

CGRP-Inp54p and Rosa-FRBPLFknockin mice successfully target their respective knockin proteins in DRG neurons. Sections from lumbar DRG from A,B) CGRP-Inp54p+/− knockin mouse stained with antibodies to GFP (cross-reacts with Venus and CFP), CGRP and fluorescent IB4-binding. Venus-FKBP12-Inp54p was confined to cell bodies of CGRP+ neurons. C) FRBPLF-CFP mouse stained with antibodies to GFP and the pan-neuronal marker NeuN. FRBPLF-CFP was localized to the membrane and axons of all neurons. Representative of two animals per condition. Scale bar, 100 μm.

Back to article page