Table 4 Primers for FKBP12 and FKBP12.6 amplification of coding exons and flanking region sequences.
From: A negative screen for mutations in calstabin 1 and 2 genes in patients with dilated cardiomyopathy
Gene | Exon | Primer | Amplicon |
|---|---|---|---|
FKBP12 | 1F | 5'- TCTCGGGGCTTCTGGGCTTC -3' | 269 |
1R | 5'- GCGCCACTACTCACCGTCTC -3' | ||
2F | 5'- CATGCCCGTCTCTGTCTCCTCA -3' | 242 | |
2R | 5'- AAGCCCCAGGCTGCCCCTGA -3' | ||
3F | 5'- TGCTGCTGGTTTCTGACTTGT -3' | 299 | |
3R | 5'- TGGCATGAGGGTGAGACTTTTC -3' | ||
4F | 5'- TTTCTTACAGAGACAGTTGGGG -3' | 253 | |
4R | 5'- GCCCTTCAGTATTCCATTTCCT -3' | ||
FKBP12.6 | 1F | 5'- GAATGGATGGATGGAGGATG -3' | 527 |
1R | 5'- TCCTAGACCCCCAAGAATCC -3' | ||
2F | 5'-CCAGAGAGGTGACGTGA-3' | 390 | |
2R | 5'-TAGACTGGCTGGGAGGA-3' | ||
3F | 5'-GGGAGTTTACTCATGACCA-3' | 368 | |
3R | 5'-CAGGGACACAAAAGAGGTA-3' | ||
4F | 5'-CAGTCACATCTCTGCTGA-3' | 510 | |
4R | 5'-CGCATGAACACTGACGAA-3' |