Table 2 Description of stemness gene primers used in quantitative PCR
From: 5-Azacytidine Is Insufficient For Cardiogenesis In Human Adipose-Derived Stem Cells
Gene | Accession No | Primer 5' 3' | PCR product size (bp) |
|---|---|---|---|
GAPDH | NM_002046 | R 5'-GGAGGAGTGGGTGTCGCTGT-3' | 217 |
F 5'-TCCCTGAGCTGAACGGGAAG-3' | |||
Sox2 | NM_003106.2 | R 5'GGTAGTGCTGGGACATGTGAA-3' | 132 |
F 5'-TTACCTCTTCCTCCCACTCCA-3' | |||
FGF4 | NM_002007 | R 5'-GGTTCCCCTTCTTGGTCTTC-3' | 118 |
F 5'-GATGAGTGCACGTTCAAGGA-3' | |||
Nanog3 | NM_024865.2 | R 5'-TGTTTGCCTTTGGGACTGGT-3' | 153 |
F 5'CTGTGATTTGTGGGCCTGAA-3' | |||
Oct-4 | NM_002701 | R 5'-GAAGTGAGGGCTCCCATAGC-3' | 180 |
F 5'-AAGGATGTGGTCCGAGTGTG-3' |