Table 1 Description of cardiogenic gene primers used in quantitative PCR
From: 5-Azacytidine Is Insufficient For Cardiogenesis In Human Adipose-Derived Stem Cells
Gene | Accession No | Primer 5' 3' | PCR product size (bp) |
|---|---|---|---|
GATA-4 | NM_002052 | R 5'-CTCCTACTCCAGCCCCTACC-3' | 207 |
F 5'-GTGGACATAGCCCCACAGTT-3' | |||
MLC-2a | NM_021223 | R 5'-GCAGACCTGAGGGAGACCTAC-3' | 135 |
F 5'-ATTGAGCTTCTCCCCAAAGAG-3' | |||
MLC-2v | NM_000432 | R 5'-ACAGGGATGGCTTCATTGAC-3' | 192 |
F 5'-ATGCGTTGAGAATGGTTTCC-3' | |||
Nkx2.5 | NM_004387 | R 5'-GAGAAGACAGAGGCGGACAA-3' | 187 |
F 5'-AGATCTTGACCTGCGTGGAC-3' | |||
β-MHC | NM_000257 | R 5'-TGATCTGGAGCTGACACTGG-3' | 196 |
F 5'-CAGGGTGTTGACCTTGTCCT-3' | |||
α-MHC | NM_002471 | R 5'-CCACCCAAGTTCGACAAGAT-3' | 127 |
F 5'-CACAGAAGAGGCCCGAGTAG-3' | |||
ANP | NM_000906 | R 5'-GCATTGAGCTGACACGAAAA-3' | 219 |
F 5'-CCTTGACGATGTCATTGGTG-3' | |||
Connexin 43 | NM_000165 | R 5'-GGACATGCACTTGAAGCAGA-3' | 103 |
F 5'-GATGATGTAGGTTCGAAGCA-3' | |||
TnIc | NM_000363 | R 5'-CTCCAACTACCGCGCTTATG-3' | 116 |
F 5'-CCAGCTCTTGCTTTGCAATC-3' | |||
TnTc | NM_000364 | R 5'-CATGGAGAAGGACCTGAATGA-3' | 108 |
F 5'-CGTCTCTCGATCCTGTCTTTG-3' | |||
MEF2C | NM_002397 | R 5'-CAGTCATTGGCTACCCCAGT-3' | 221 |
F 5'-GCAGATGGTGGCATGTTATG-3' | |||
Desmin | NM_001927 | R 5'-GCCTCATCAGGGAATCGTTA-3' | 129 |
L 5'-AGGCAGCCAACAAGAACAAC-3' | |||
α-Actin | NM_005159 | R 5'-AGGGGCTGGAAGAGTGTCTCA-3' | 136 |
L 5' -GCCCTGGATTTTGAGAATGA-3' |